Enhancing Student Support in Higher Education: Occupation-Based Programming and the Vital Role of Occupational Therapy

Introduction: With the higher education student populations facing greater prevalence of mental health conditions and greater presence of students with disabilities attending higher education, there is a greater demand than many higher education resources can meet. Therefore, there is a need to revise the current student support structure. This project advocated for the role of occupational therapy (OT) program development in higher education student disability services. Purpose: The primary aim of this project was to develop, implement, and evaluate a virtual 4-week occupation-based pilot program. The initial pilot participants included students receiving accommodations from the Student Accessibility and Accommodations (SA&A) Office within all OT programs at St. Catherine University. The focus of the program was to enhance occupational balance via education and accountability of academic and non-academic skills, such as time management, study skills, and mindfulness. Approach: An extensive literature search, two scoping reviews, review of existing survey data, and a needs assessment was conducted to gather background information prior to program implementation. Outcomes: Nine participants registered for the program. Although there was some attrition, adequate participation was achieved to receive feedback via the program surveys for future program revision and re-implementation. Overall, participants stated several program strengths, and changes in occupational balance and behaviors associated with time management were noted. Recommendations: The SA&A Office and the OT profession can continue this this partnership in developing creative initiatives, such as programming, to support students

The genetics of virus particle shape in equine influenza A virus

Background Many human strains of influenza A virus produce highly pleomorphic virus particles that at the extremes can be approximated as either spheres of around 100 nm diameter or filaments of similar cross-section but elongated to lengths of many microns. The role filamentous virions play in the virus life cycle remains enigmatic. Objectives/Methods Here, we set out to define the morphology and genetics of virus particle shape in equine influenza A virus, using reverse genetics and microscopy of infected cells. Results and Conclusions The majority of H3N8 strains tested were found to produce filamentous virions, as did the prototype H7N7 A/eq/Prague/56 strain. The exception was the prototype H3N8 isolate, A/eq/Miami/63. Reassortment of equine influenza virus M genes from filamentous and non-filamentous strains into the non-filamentous human virus A/PR/8/34 confirmed that segment 7 is a major determinant of particle shape. Sequence analysis identified three M1 amino acid polymorphisms plausibly associated with determining virion morphology, and the introduction of these changes into viruses confirmed the importance of two: S85N and N231D. However, while either change alone affected filament production, the greatest effect was seen when the polymorphisms were introduced in conjunction. Thus, influenza A viruses from equine hosts also produce filamentous virions, and the major genetic determinants are set by the M1 protein. However, the precise sequence determinants are different to those previously identified in human or porcine viruses

Work Participation Interventions for Individuals with Disabilities: An Evidence-Based Practice Project

This Evidence-Based Practice (EBP) project considered the following question: What are the characteristics of interventions, programs, and services that are effective in supporting work participation for individuals with disabilities and their employers

Budding of filamentous and non-filamentous influenza A virus occurs via a VPS4 and VPS28-independent pathway

The mechanism of membrane scission during influenza A virus budding has been the subject of controversy. We confirm that influenza M1 binds VPS28, a subunit of the ESCRT-1 complex. However, confocal microscopy of infected cells showed no marked colocalisation between M1 and VPS28 or VPS4 ESCRT proteins, or relocalisation of the cellular proteins. Trafficking of HA and M1 appeared normal when endosomal sorting was impaired by expression of inactive VPS4. Overexpression of either isoform of VPS28 or wildtype or dominant negative VPS4 proteins did not alter production of filamentous virions. SiRNA depletion of endogenous VPS28 had no significant effect on influenza virus replication. Furthermore, cells expressing wildtype or dominant-negative VPS4 replicated filamentous and non-filamentous strains of influenza to similar titres, indicating that influenza release is VPS4-independent. Overall, we see no role for the ESCRT pathway in influenza virus budding and the significance of the M1-VPS28 interaction remains to be determined. (C) 2009 Elsevier Inc. All rights reserved